Phosphate buffered saline, pH 7.4, containing 0.02% Tween 20 (PBS-T):

• NaCl 8.00g
• KH2PO4 0.20g
• Na2HPO4 1.15g
• KCl 0.20g
• Tween 20 0.2ml

All made up to 1 liter in deionized water. Add 1g bovine serum albumin (BSA, Sigma A-7030) to 100ml PBS-T to make 1% BSA PBS-T.
Sterile filter.